Antioxidant Activity of Ethanol Extract Combination of Cavendis Banana Peel (Musa acuminata) and Kepok Banana Peel (Musa paradisiaca) Using the DPPH Method

Authors

  • Adriyan Suhada Program Studi D-III Farmasi, Universitas Bima Internasional MFH
  • Tuhfatul Ulya Program Studi Pendidikan Profesi Apoteker, FKIK, Universitas Mataram
  • Roushandy Asri Fardani Program Studi D-III Teknologi Laboratorium Medis, Universitas Bima Internasional MFH
  • Ajeng Dian Pertiwi Program Studi D-III Farmasi, Universitas Bima Internasional MFH
  • Idham Halid Program Studi D4 Keselamatan dan Kesehatan Kerja, Universitas Bima Internasional MFH
  • Pauzan Pauzan Program Studi D-III Teknologi Laboratorium Medis, Universitas Bima Internasional MFH
  • Wulan Ratia Ratulangi Program Studi D-III Farmasi, Universitas Bima Internasional MFH
  • Nurul Indriani Program Studi S1 Farmasi, Universitas Bumi Gora
  • Ni Nyoman Ariwidiani Program Studi D4 Teknologi Laboratorium Medis, Universitas Bima Internasional MFH
  • Rosnalia Widyan Program Studi D-III Farmasi, Universitas Bima Internasional MFH
  • Evi Fatmi Utami Program Studi D-III Farmasi, Universitas Bima Internasional MFH
  • Wanda Novianty Program Studi D-III Farmasi, Universitas Bima Internasional MFH

DOI:

https://doi.org/10.33394/j-ps.v13i3.15782

Keywords:

antioxidant, cavendis banana peel, kepok banana peel, IC50, DPPH

Abstract

Banana peels, often considered agricultural waste, are rich in bioactive compounds with significant antioxidant potential. This study evaluates the antioxidant activity of a combined ethanol extract from Cavendis (Musa acuminata) and Kepok (Musa paradisiaca) banana peels using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The extraction was performed via maceration with 96% ethanol, yielding 9.13%. Phytochemical analysis identified key compounds including flavonoids, tannins, saponins, and steroids/terpenoids, which are known to exhibit antioxidant properties. The extract demonstrated strong radical scavenging activity with an IC50 of 6.47 ppm and an Antioxidant Activity Index (AAI) of 5.40, comparable to Vitamin C (IC50 = 6.29 ppm; AAI = 5.56). These results indicate a synergistic effect from combining the two banana peel varieties, enhancing antioxidant efficacy beyond that reported for individual peels. This study supports the potential use of Cavendis and Kepok banana peel extracts as sustainable natural antioxidants for application in food preservation, nutraceuticals, and cosmetics. Future work should focus on optimizing extraction methods, detailed phytochemical profiling, stability assessment, and in vivo validation to advance product development and maximize health benefits.

References

Aly, A., Tahoon, N., & Faid, J. (2017). Comparative study between synthetic and natural antioxidants of banana, mango, and orange peels extracts and their effect on the soybean and olive oils. Journal of Food and Dairy Sciences, 8(8), 347–352. https://doi.org/10.21608/jfds.2017.38898

Anjum, S., Mishra, P., & Sundaram, S. (2022). Nutraceutical application and industrial utilization of banana (Musa paradisica L. variety “Bhusawal Keliâ€) peels (Review article). International Journal of Pharmacy and Pharmaceutical Sciences, 14–20. https://doi.org/10.22159/ijpps.2022v14i2.43211

Arista, N., & Siregar, R. (2023). Antioxidant activity test of Barangan banana peel (Musa acuminata Linn) ethanol extract with DPPH method. Indonesian Journal of Chemical Science and Technology (IJCST), 6(2), 171. https://doi.org/10.24114/ijcst.v6i2.49373

Bassuony, N. (2015). Chemical composition, antioxidant, and antibacterial activities of ethanolic banana peel and lemon seed extracts. Journal of Agricultural Chemistry and Biotechnology, 6(6), 167–175. https://doi.org/10.21608/jacb.2015.44294

Hidayati, J., Yudiati, E., Pringgenies, D., Oktaviyanti, D., & Kusuma, A. (2020). Comparative study on antioxidant activities, total phenolic compound, and pigment contents of tropical Spirulina platensis, Gracilaria arcuata, and Ulva lactuca extracted in different solvents polarity. E3S Web of Conferences, 147, 03012. https://doi.org/10.1051/e3sconf/202014703012

Khacharat, L., Mahakarnchanakul, W., Sukatta, U., Sae-tan, S., Rugthaworn, P., Klinsukhon, K., & Sakayaroj, S. (2022). Effect of banana peels and phenolic compounds on pigments and citrinin production by Monascus purpureus. Agriculture and Natural Resources, 56(1), 203–214. https://li01.tci-thaijo.org/index.php/anres/article/view/254017

Khamsaw, P. (2024). Banana peel (Musa ABB cv. Nam Wa Mali-Ong) as a source of value-adding components and the functional properties of its bioactive ingredients. Plants, 13(5), 593. https://doi.org/10.3390/plants13050593

Khang, Ä., Tien, L., Men, T., & Thuy, N. (2021). Potential of fermented fruit peel liquid in cosmetics as a skin care agent. Cosmetics, 8(2), 33. https://doi.org/10.3390/cosmetics8020033

Khoozani, A., Birch, J., & Bekhit, A. (2019). Production, application, and health effects of banana pulp and peel flour in the food industry. Journal of Food Science and Technology, 56(2), 548–559. https://doi.org/10.1007/s13197-018-03562-z

Kumari, P. (2023). Banana and its byâ€products: A comprehensive review on its nutritional composition and pharmacological benefits. Efood, 4(5). https://doi.org/10.1002/efd2.110

Lopes, S., Galani, G., Peruch, L., & Maraschin, M. (2022). In vitro determination of the sun protection factor (SPF) and photochemoprotection. Journal Name (lengkapi informasi jika ada)

Nofianti, T., Muhtadi, A., & Fidrianny, I. (2021). Comparison of antihyperglycemic activity of different parts of Klutuk banana (Musa balbisiana Colla). International Journal of Applied Pharmaceutics, 57–61. https://doi.org/10.22159/ijap.2021.v13s3.12

Okolie, J., Henry, O., & Epelle, E. (2016). Determination of the antioxidant potentials of two different varieties of banana peels in two different solvents. Food and Nutrition Sciences, 7(13), 1253–1261. https://doi.org/10.4236/fns.2016.713115

Parvez, G., Tonu, J., Ara, R., Joarder, M., Milon, M., Sarker, R., … & Kader, M. (2023). Phytochemical and antioxidant comparison of different varieties of banana. Journal of Pharmacognosy and Phytochemistry, 12(1), 194–199. https://doi.org/10.22271/phyto.2023.v12.i1c.14574

Pereira, G., Arruda, H., & Pastore, G. (2016). Optimizing the homogenizerâ€assisted extraction (HAE) of total phenolic compounds from banana peel. Journal of Food Process Engineering, 40(3). https://doi.org/10.1111/jfpe.12438

Rahmi, A., Hardi, N., & Hevira, L. (2022). Aktivitas antioksidan ekstrak kulit pisang kepok, pisang mas, dan pisang nangka menggunakan metode DPPH. JIFFK Jurnal Ilmu Farmasi dan Farmasi Klinik, 18(2), 77. https://doi.org/10.31942/jiffk.v18i2.5961

Samiasih, A., Khoiriyah, K., Norma, E., Rahmawati, S., Pranata, S., & Rino, V. (2023). The gastroprotective role of yellow Kepok banana (Musa x paradisiaca L. var. Kepok) peel extract and influence on markers of oxidative stress: Malondialdehyde and nitric oxide. Scripta Medica, 54(1), 53–59. https://doi.org/10.5937/scriptamed54-42580

Šeremet, D., Durgo, K., Komljenović, A., Antolić, M., Jarić, A., Turković, A., … & Šantek, B. (2022). Red beetroot and banana peels as value-added ingredients: Assessment of biological activity and preparation of functional edible films. Polymers, 14(21), 4724. https://doi.org/10.3390/polym14214724

Siji, S., & P.V., N. (2017). Antioxidants and antioxidant activity common in eight banana varieties in Kerala. International Journal of Advanced Engineering Research and Science, 4(7), 118–123. https://doi.org/10.22161/ijaers.4.7.18

Sutjiatmo, A., Widowati, W., Sumiati, I., … & Azizah, A. (2021). Antioxidant and anticancer potential of Raja Bulu banana peel and heart ethanol extracts in MCF-7 cell lines. Majalah Obat Tradisional, 26(1), 49. https://doi.org/10.22146/mot.55333

Türker, B., & Savlak, N. (2021). Gluten-free cake with unripe banana peel flour substitution: Impact on nutritional, functional, and sensorial properties. Nutrition & Food Science, 52(6), 980–995. https://doi.org/10.1108/nfs-08-2021-0259

Vu, H., Scarlett, C., & Vuong, Q. (2016). Optimization of ultrasound-assisted extraction conditions for recovery of phenolic compounds and antioxidant capacity from banana (Musa cavendish) peel. Journal of Food Processing and Preservation, 41(5), e13148. https://doi.org/10.1111/jfpp.13148

Vu, H., Scarlett, C., & Vuong, Q. (2019). Maximising recovery of phenolic compounds and antioxidant properties from banana peel using microwave-assisted extraction and water. Journal of Food Science and Technology, 56(3), 1360–1370. https://doi.org/10.1007/s13197-019-03610-2

Yunus, Z., Asman, S., & AL, N. (2020). Investigation of absorbent, antioxidant, and thickening agent properties of tropical fruit peels. Journal of Sustainability Science and Management, 15(8), 63–79. https://doi.org/10.46754/jssm.2020.12.006

Downloads

Published

2025-07-01

How to Cite

Suhada, A., Ulya, T., Fardani, R. A., Pertiwi, A. D., Halid, I., Pauzan, P., … Novianty, W. (2025). Antioxidant Activity of Ethanol Extract Combination of Cavendis Banana Peel (Musa acuminata) and Kepok Banana Peel (Musa paradisiaca) Using the DPPH Method. Prisma Sains : Jurnal Pengkajian Ilmu Dan Pembelajaran Matematika Dan IPA IKIP Mataram, 13(3), 769–781. https://doi.org/10.33394/j-ps.v13i3.15782

Issue

Section

Research Articles