Potensi Simplisia Biji Kemangi (Ocimum Basilicum) Pada Pakan Terhadap Histomorfometri dan Kerusakan Otot Ikan Lele (Clarias Gariepinus) Akibat Paparan Mikroplastik Polistiren
DOI:
https://doi.org/10.33394/bioscientist.v14i1.19297Keywords:
Biji kemangi, degenerasi, mikroplastik PS, Myofiber, nekrosisAbstract
This study aims to analyze the potential of basil seed simplicia in feed to improve histomorphometric and muscle damage of catfish due to exposure to PS microplastics. This study is an experimental type using a Completely Randomized Design (CRD) with five treatments, namely K (0 mg/L PS microplastic + 0% basil seed simplicia), KP (0 mg/L PS microplastic + 2% basil seed simplicia), KN (100 mg/L PS microplastic + 0% basil seed simplicia), P1 (100 mg/L PS microplastic + 2% basil seed simplicia), P2 (100 mg/L PS microplastic + 4% basil seed simplicia). Microplastic exposure was carried out on 125 catfish for 15 days, the parameters observed included myofiber diameter, myofiber area, degeneration, and muscle cell necrosis. The results showed that exposure to PS microplastics significantly increased the diameter and area of myofibers and reduced degeneration and necrosis of catfish muscle cells (P<0.05). The administration of 2% basil seed simplicia (P1) in the feed was able to increase the diameter and area of myofibers and reduce the level of degeneration and necrosis significantly compared to KN, while the administration of 4% basil seed simplicia (P2) showed lower effectiveness. The conclusion of this study shows that 2% basil seed simplicia (P1) has the potential to improve histomorphometrics and muscle damage in catfish due to exposure to polystyrene microplastics.
References
Alif, A., Syawal, H., & Riauwaty, M. (2021). Histopatologi Hati dan Usus Ikan Jambal Siam (Pangasionodon hypophthalmus) yang Diberi Pakan Mengandung Ekstrak Daun Rhizophora apiculata. Jurnal Ilmu Perairan (Aquatic Science), 9(2), 152–161. https://jipas.ejournal.unri.ac.id/index.php/jipas/article/view/1992
Amar, M. I., Martana, B., Rizal, R., & Hidayati, A. N. (2022). Peningkatan Pengetahuan dan Keterampilan Masyarakat Tentang Pengolahan Hasil Budidaya Ikan Lele Melalui Diversifikasi pada Olahan Ikan Lele. JMM (Jurnal Masyarakat Mandiri), 6(2), 1340. https://doi.org/10.31764/jmm.v6i2.7204
Bravo, H. C., Cespedes, N. V., Zura-Bravo, L., & Munoz, L. A. (2021). Basil Seeds as A Novel Food, Source of Nutrients and Functional Ingredients With Beneficial Properties: A Review. Foods, 10(7), 1–18. https://doi.org/10.3390/foods10071467
Cai, W., Wang, X., Liu, H., Hua, L., Han, D., Zhu, X., Jin, J., Zhang, Z., Yang, Y., & Xie, S. (2025). Dietary Supplementation with Glutamate Improves the Flesh Quality of Gibel Carp (Carassius gibelio) by Altering Muscle Texture Characteristics and Increasing the Deposition of Flavour Substances. Journal of Nutritional Science, 14, 1. https://doi.org/10.1017/jns.2025.10009
Chen, G., Li, Y., & Wang, J. (2021). Occurrence and Ecological Impact of Microplastics in Aquaculture Ecosystems. Chemosphere, 274, 1–9. https://doi.org/10.1016/j.chemosphere.2021.129989
EFSA. (2016). Presence of Microplastics and Nanoplastics in Food, with Particular Focus on Seafood. EFSA Journal, 14(6), 1–30. https://doi.org/10.2903/j.efsa.2016.4501
El-Dakar, A. Y., Shalaby, S. M., Nemetallah, B. R., Saleh, N. E., Sakr, E. M., & Toutou, M. M. (2015). Possibility of Using Basil (Ocimum basilicum) Supplementation in Gilthead Sea Bream (Sparus aurata) Diet. Egyptian Journal of Aquatic Research, 41(2), 203–210. https://doi.org/10.1016/j.ejar.2015.03.001
Filho, J. V. D., Pedroti, V. P., Santos, B. L. T., Pinheiro, M. M. de L., Mira, Á. B. de, Silva, F. C. da, Silva, E. C. S. e, Cavali, J., Guedes, E. A. C., & Schons, S. de V. (2023). First Evidence of Microplastics in Freshwater from Fish Farms in Rondônia State, Brazil. Heliyon, 9(4), 1–11. https://doi.org/10.1016/j.heliyon.2023.e15066
Firnanda, W. A., Kisnarti, E. A., & Permatasari, I. N. (2024). Kajian Distribusi Mikroplastik dan Analisis Polimer pada Sedimen dan Air Laut (Literature Review). J-Tropimar, 6(2), 103–114. https://doi.org/https://doi.org/10.30649/jrkt.v6i2.85
Haetami, K., Erdiasari, E., Intan Pratama, R., Grandiosa Herman, R., Studi Perikanan, P., Perikanan dan Ilmu Kelautan, F., & Padjadjaran Jl Raya Bandung-Sumedang, U. K. (2023). Effect of Addition of Turmeric Extract (Curcuma domestica) on Feed to Growth and Survival Dumbo Catfish Seeds (Clarias gariepinus). Journal Perikanan, 13(4), 1111–1121. https://doi.org/10.29303/jp.v13i3.657
Hamed, M., Soliman, H. A. M., Badrey, A. E. A., & Osman, A. G. M. (2021). Microplastics Induced Histopathological Lesions in Some Tissues of Tilapia (Oreochromis niloticus) Early Juveniles. Tissue and Cell, 71, 1–9. https://doi.org/10.1016/j.tice.2021.101512
Hamed, M., Soliman, H. A. M., Osman, A. G. M., & Sayed, A. E. D. H. (2020). Antioxidants and Molecular Damage in Nile Tilapia (Oreochromis niloticus) after Exposure to Microplastics. Environmental Science and Pollution Research, 27(13), 14581–14588. https://doi.org/10.1007/s11356-020-07898-y
Jiang, J., Wu, X. Y., Zhou, X. Q., Feng, L., Liu, Y., Jiang, W. D., Wu, P., & Zhao, Y. (2016). Glutamate Ameliorates Copper-Induced Oxidative Injury by Regulating Antioxidant Defences in Fish Intestine. British Journal of Nutrition, 116(1), 70–79. https://doi.org/10.1017/S0007114516001732
Jones, J. I., Vdovchenko, A., Cooling, D., Murphy, J. F., Arnold, A., Pretty, J. L., Spencer, K. L., Markus, A. A., Vethaak, A. D., & Resmini, M. (2020). Systematic Analysis of the Relative Abundance of Polymers Occurring as Microplastics in Freshwaters and Estuaries. International Journal of Environmental Research and Public Health, 17(24), 1–12. https://doi.org/10.3390/ijerph17249304
Kama, N. A., Djawad, M. I., & Yaqin, K. (2024). Effects of Microplastics on the Oxygen Consumption and Histological Changes of the Cultured Nile Tilapia Oreochromis Niloticus. Egyptian Journal of Aquatic Biology & Fisheries, 28(5), 945–960. https://doi.org/10.21608/ejabf.2024.382106
Kelautan, K., & Perikanan, D. (2021). Laporan Kinerja Tahun 2021. Pemerintah Daerah Kota Jakarta, Provinsi DKI Jakarta.
Liu, G., Ito, T., Kijima, Y., Yoshitake, K., Asakawa, S., Watabe, S., & Kinoshita, S. (2022). Zebrafish Danio Rerio Myotomal Muscle Structure and Growth from a Spatial Transcriptomics Perspective. Genomics, 114(5). https://doi.org/10.1016/j.ygeno.2022.110477
Mahmud, F., Sarker, D. B., Jocelyn, J. A., & Sang, Q. X. A. (2024). Molecular and Cellular Effects of Microplastics and Nanoplastics: Focus on Inflammation and Senescence. Cells 13(21).1788. https://doi.org/10.3390/cells13211788
Marpaung, M. P., & Romelan. (2018). Analisis Jenis dan Kadar Saponin Ekstrak Metanol Daun Kemangi (Ocimum basilicum L.) dengan Menggunakan Metode Gravimetri. JFL Jurnal Farmasi Lampung, 07(2), 81–86. https://doi.org/https://doi.org/10.37090/jfl.v7i2.57
Nazir, S., & Wani, I. A. (2021). Physicochemical Characterization of Basil (Ocimum basilicum L.) Seeds. Journal of Applied Research on Medicinal and Aromatic Plants, 22. https://doi.org/10.1016/j.jarmap.2021.100295
Nurdhiana, I. (2021). Mikroplastik pada Ikan Mujair (Oreochromis mossambicus) di Keramba Ikan Kali Kanal Mangetan Kabupaten Sidoarjo. Environmental Pollution Journal, 3(3), 192–198. https://doi.org/https://doi.org/10.58954/epj.v1i3.68
Omnes, M. H., Le Goasduff, J., Le Delliou, H., Le Bayon, N., Quazuguel, P., & Robin, J. H. (2017). Effects of Dietary Tannin on Growth, Feed Utilization and Digestibility, and Carcass Composition in Juvenile European Seabass (Dicentrarchus labrax L.). Aquaculture Reports, 6, 21–27. https://doi.org/10.1016/j.aqrep.2017.01.004
Pandu Wicaksana, M., & Firmani, U. (2025). Kelimpahan dan Jenis Mikroplastik pada Tambak Budidaya Sistem Polikultur di Kecamatan Duduk sampeyan Kabupaten Gresik. Jurnal Perikanan Pantura (JPP), 8(1), 653–660. https://doi.org/https://doi.org/10.30587/jpp.v8i1.9457
Sawalman, R., Zamani, N. P., Werorilangi, S., & Ismet, M. S. (2021). Akumulasi Mikroplastik pada Spesies Ikan Ekonomis Penting di Perairan Pulau Barranglompo, Makassar. Jurnal Ilmu dan Teknologi Kelautan Tropis, 13(2), 241–259. https://doi.org/10.29244/jitkt.v13i2.34587
Sayed, A. E. D. H., Hana, M. N., Hamed, M., Abdel-Latif, H. M. R., Lee, J. S., & Soliman, H. A. M. (2023). Protective Efficacy of Dietary Natural Antioxidants on Microplastic Particles-Induced Histopathological Lesions in African Catfish (Clarias gariepinus). Environmental Science and Pollution Research, 30(9), 24424–24440. https://doi.org/10.1007/s11356-022-23789-w
Simanjutak H, A. (2021). Studi Pemanfaatan Tumbuhan Obat Antidiare oleh Masyarakat di Etnis Sumatera Utara. Herbal Medicine Journal, 4(1), 1–12. https://doi.org/https://doi.org/10.58996/hmj.v4i2.32
Suwartiningsih, N., Aszhara, A. F., Marsela, R., & Wijayanti, D. E. (2025). The Effect of Polystyrene Microplastic Exposure in the Rearing Water on Muscle Morphology of Mutiara Catfish (Clarias gariepinus Burchell, 1822). Jurnal Penelitian Pendidikan IPA, 11(7), 295–302. https://doi.org/10.29303/jppipa.v11i7.11537
Wiranantha, I. G., Setyawati, I., & Wiratmini, N. I. (2019). Histopatologi serta Aktivitas Hati Kelinci Lokal (Lepus sp.) yang Diberi Ransum Tepung Daun Kaliandra (Calliandra calothyrsus Meissn.) dan Kulit Nanas (Ananas comosus L.). Metamorfosa: Journal of Biological Sciences, 6(2), 183–190. https://doi.org/10.24843/metamorfosa.v06.i02.p07
Yuriana, L., Santoso, H., & Sutanto, A. (2017). Pengaruh Probiotik Strain Lactobacillus Terhadap Laju Pertumbuhan dan Efisiensi Pakan Lele Masamo (Clarias Sp) Tahap Pendederan I dengan Sistem Bioflok Sebagai Sumber Biologi. Jurnal Lentera Pendidikan Pusat Penelitian LPPM UM METRO, 2(1), 13–23. https://doi.org/http://dx.doi.org/10.24127/jlplppm.v2i1.476
Yusuf, M. A., & Effendi, I. (2024). Evaluasi Dampak Pencemaran Mikroplastik pada Sistem Budidaya Perikanan: Strategi Pengelolaan Berkelanjutan. e-journal Budidaya Perairan, 12(2), 92–101. https://doi.org/https://doi.org/10.35800/bdp.v12i2.59436
Zhao, Y., Jiang, Q., Zhou, X. Q., Xu, S. X., Feng, L., Liu, Y., Jiang, W. D., Wu, P., Zhao, J., & Jiang, J. (2020). Effect of Dietary Threonine on Growth Performance and Muscle Growth, Protein Synthesis and Antioxidant-Related Signalling Pathways of Hybrid Catfish Pelteobagrus Vachelli♀ × Leiocassis Longirostris♂. British Journal of Nutrition, 123(2), 121–134. https://doi.org/10.1017/S0007114519002599
Ziolkowska, E., Bogucka, J., Rawski, M., Mazurkiewicz, J., Maiorano, G., & Stanek, M. (2022). The First Insights on Trans-Galactooligosaccharide Effects on Fatty Acids Profile and Microstructure of Muscle in Common Carp. Annals of Animal Science, 22(1), 305–324. https://doi.org/10.2478/aoas-2021-0030
Zulfadhli, Wijayanti, N., & Retnoaji, B. (2016). Perkembangan Ovarium Ikan Wader Pari (Rasbora Lateristriata Bleeker, 1854): Pendekatan Histologi. Jurnal Perikanan Tropis, 3(1), 1–8. https://doi.org/https://doi.org/10.35308/jpt.v3i1.34
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