Isolation and Characterization of Endophytic Bacteria from Curcuma Aeruginosa Roxb As Antibacterial Candidates Againts Pneumonia

Authors

  • Arti Wahyu Utami Sekolah Tinggi Ilmu Kesehatan Tujuh Belas
  • Firman Rezaldi Sekolah Tinggi Ilmu Kesehatan Tujuh Belas
  • Rifkarosita Putri Ginaris Sekolah Tinggi Ilmu Kesehatan Tujuh Belas
  • Jihan Nabilla Sekolah Tinggi Ilmu Kesehatan Tujuh Belas
  • Bramantyo Sekolah Tinggi Ilmu Kesehatan Tujuh Belas
  • Puput Setiawati Sekolah Tinggi Ilmu Kesehatan Tujuh Belas
  • Zulfania Shandra Fatikha Sekolah Tinggi Ilmu Kesehatan Tujuh Belas
  • Nanik Pujiastuti Dinas Kesehatan Kabupaten Karanganyar
  • Dwi Wahyuni Institut Ilmu Kesehatan Bhakti Wiyata Kediri

DOI:

https://doi.org/10.33394/bioscientist.v13i4.18148

Keywords:

Isolation, characterization, endophytic bacteria, pneumonia

Abstract

This study aimed to isolate and characterize endophytic bacteria from black turmeric rhizomes (Curcuma aeruginosa Roxb) and test their potential as antibacterial agents against Streptococcus pneumoniae. This experimental research began with the isolation of endophytic bacteria from surface-sterilized black turmeric rhizomes. The obtained pure isolates were characterized macroscopically (colony shape, margin, elevation, pigment) and microscopically (cell shape, Gram staining). The growth curve of potential isolates was determined, and antibacterial activity was tested using the disc diffusion method against S. pneumoniae, with ciprofloxacin as a positive control. Six endophytic bacterial isolates (PS1, PS2, PS3, PS4, PS5, PS6) were successfully isolated, showing variations in colony morphology, and all were classified as Gram-negative bacilli. All isolates exhibited strong antibacterial activity against Streptococus pneumoniae, with inhibition zone diameters ranging from 15.3 mm to 20.6 mm. Black turmeric rhizomes (Curcuma aeruginosa Roxb) harbor diverse endophytic bacteria with significant potential as antibacterial agents against the pneumonia pathogen, Streptococcus pneumoniae.

References

Ali, A., Ahmed, T., Ibrahim, E., Rizwan, M., Phin, K., Wan, J., & Yong, H. (2024). Heliyon Review Article A Review On Mechanisms And Prospects Of Endophytic Bacteria In Biocontrol Of Plant Pathogenic Fungi And Their Plant Growth-Promoting Activities. Heliyon, 10(11), e31573. https://doi.org/10.1016/j.heliyon.2024.e31573

Amari, S., Warda, K., Bouraddane, M., Katfy, M., Elkamouni, Y., Arsalane, L., Zerouali, K., Zouhair, S., & Bouskraoui, M. (2023). Antibiotic Resistance of Streptococcus pneumoniae in the Nasopharynx of Healthy Children Less than Five Years Old after the Generalization of Pneumococcal Vaccination in Marrakesh, Morocco. Antibiotics, 12(3). https://doi.org/10.3390/antibiotics12030442

Apriyanthi. (2022). Identifikasi Bakteri Kontaminan Pada Gelang Tri Datu. Jurnal Biologi Makassar, 7(2), 24–33. https://journal.unhas.ac.id/index.php/bioma

Ezeobiora, C. E., Igbokwe, N. H., Amin, D. H., & Mendie, U. E. (2021). Endophytic Microbes From Nigerian Ethnomedicinal Plants: A Potential Source For Bioactive Secondary Metabolites—A Review. Bulletin of the National Research Centre, 45(1). https://doi.org/10.1186/s42269-021-00561-7

Handayani, N., Sabdaningsih, A., Jati, O. E., & Ayuningrum, D. (2023). Isolasi dan Karakterisasi Bakteri Endofit dari Akar Avicennia marina di Kawasan Mangrove Pantai Tirang, Semarang. Jurnal Pasir Laut, 7(2), 68–73. https://doi.org/10.14710/jpl.2023.59064

Hutchings, M., Truman, A., & Wilkinson, B. (2019). Antibiotics: past, present and future. Current Opinion in Microbiology, 51(Figure 1), 72–80. https://doi.org/10.1016/j.mib.2019.10.008

Irvanita, D. A., Rohmantin, D., Adz-dzikir, F., Puspita, T., Jinan, R. F., & Aji, O. R. (2024). Antibacterial Activity of Endophytic Fungi Isolated from Turmeric Plants ( Curcuma longa L .) Against Staphylococcus aureus and Escherichia coli. 4(1), 8–13.

Manna, S., Ortika, B. D., Werren, J. P., Pell, C. L., Gjuroski, I., Lo, S. W., Hinds, J., Tundev, O., Dunne, E. M., Gessner, B. D., Russell, F. M., Mulholland, E. K., Mungun, T., von Mollendorf, C., Bentley, S. D., Hilty, M., Ravenscroft, N., & Satzke, C. (2025). Streptococcus pneumoniae serotype 33H: a novel serotype with frameshift mutations in the acetyltransferase gene wciG. Pneumonia 2025 17:1, 17(1), 1–9. https://doi.org/10.1186/S41479-025-00162-2

Novita, Dede Mahdiyah, Mia Audina, & Putri Vidiasari Darsono. (2023). Aktivitas Antibakteri Dari Bakteri Tanah Gambut TerhadapStreptococcus pneumoniae. Innovative: Journal Of Social Science Research, 3(6), 8129–8141. https://j-innovative.org/index.php/Innovative/article/view/7403

Riyanto, A., & Megasari, M. (2021). Pneumonia pada Balita Tidak Diberikan ASI Eksklusif dan Imunisasi DPT-HB-HIB. Jik Jurnal Ilmu Kesehatan, 5(2), 197. https://doi.org/10.33757/jik.v5i2.420

Rizqoh, D., Kumala, W. O., Sipriyadi, S., Sinuhaji, B., & Oktoviani, O. (2021). Potensi Bakteri Endofit Andaliman (Zanthoxylum Acanthopodium DC.) Menghambat Bakteri Penyebab Infeksi pada Manusia. JUMANTIK (Jurnal Ilmiah Penelitian Kesehatan), 6(3), 194. https://doi.org/10.30829/jumantik.v6i3.8866

Sains, F., Patompo, U., & Jumrah, E. (2024). Potensi Bakteri Endofit Tanaman Penghasil Senyawa Bioaktif Jurnal SAINTEK Patompo. 2(2), 119–123.

Sari, A. P., & Supratman, U. (2022). Phytochemistry and Biological Activities of Curcuma aeruginosa ( Roxb .). 22(2),576-598. https://doi.org/10.22146/ijc.70101

Sari, E., & Bahrina, I. (2024). Hubungan Kebiasaan Merokok Dengan Kejadian Infeksi Saluran Pernafasan Akut (ISPA) Pada Balita Di Wilayah Kerja Puskesmas Langsa Timur Kota Langsa. Jurnal Wellness, 1(1), 2022–2025.

Sari, R. P., & Farmasi, F. (2024). Identifikasi Senyawa Flavonoid Dan Uji Antioksidan Ekstrak Etanol Rimpang Temu Hitam ( Curcuma aeruginoa Roxb ) Dengan Metode DPPH. 7(1), 2032–2038.

Singh, M., Kumar, A., Singh, R., & Deo, K. (2017). Endophytic Bacteria : A New Source Of Bioactive Compounds. 3 Biotech, 2, 315. https://doi.org/10.1007/s13205-017-0942-z

Sonartra, N. E., Neherta, M., Deswita, Novrianda, D., & Fajriah, L. (2024). Gambaran Perilaku Keluarga Dengan Efikasi Diri Berbeda yang Mempunyai Balita Tentang Pencegahan Pneumonia. Jurnal Ilmiah Permas: Jurnal Ilmiah STIKES Kendal, 14(2), 75–82.

Sukandiarsyah, F., Purwaningsih, I., & Ratnawaty, G. J. (2023). Aktivitas Antioksidan Ekstrak Metanol dan n-Heksana Rimpang Temu Ireng ( Curcuma aeruginosa Roxb .) Metode DPPH. 9(1), 62–70. https://doi.org/10.35311/jmpi.v9i1.299

Yunita, M., Ohiwal, M., Dirks, C. S., Angkejaya, O. W., Sukmawati, S., & Ilsan, N. A. (2022). Endophytic bacteria associated with Myristica fragrans: Improved media, bacterial population, preliminary characterization, and potential as antibacterials. Biodiversitas, 23(8), 4047–4054. https://doi.org/10.13057/biodiv/d230824

Zulfarina, Z., Rosiana, Y., Ayudia, D., & Darmawati, D. (2022). Isolasi Bakteri Endofit Dari Tanaman Laban (Vitex Pubescens Vahl) Sebagai Antibakteri. JST (Jurnal Sains Dan Teknologi), 11(1), 85–92. https://doi.org/10.23887/jstundiksha.v11i1.42781

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Published

2025-12-01

How to Cite

Utami, A. W., Rezaldi, F., Ginaris, R. P., Nabilla, J., Bramantyo, Setiawati, P., … Wahyuni, D. (2025). Isolation and Characterization of Endophytic Bacteria from Curcuma Aeruginosa Roxb As Antibacterial Candidates Againts Pneumonia. Bioscientist : Jurnal Ilmiah Biologi, 13(4), 2648–2656. https://doi.org/10.33394/bioscientist.v13i4.18148

Issue

Vol. 13 No. 4 (2025): December

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Articles

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Copyright (c) 2025 Arti Wahyu Utami, Firman Rezaldi, Rifkarosita Putri Ginaris, Jihan Nabilla, Bramantyo, Puput Setiawati, Zulfania Shandra Fatikha, Nanik Pujiastuti, Dwi Wahyuni

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