Isolasi dan Identifikasi Bakteri Lipolitik Indigenous untuk Bioremediasi Tanah Limbah Pabrik Kelapa Sawit
DOI:
https://doi.org/10.33394/bioscientist.v13i4.17644Keywords:
Bakteri lipolitik, bioremediasi, pelepah daun kelapa sawit, limbah cair kelapa sawit, degradasi lemakAbstract
This study aims to isolate, identify, and test the potential of native lipolytic bacteria from LCPKS contaminated soil, as well as to determine the effect of supplementation of oil palm leaf midrib extract on its degradation activity. The research method uses an experimental design with isolation techniques and morphological characterization of bacteria, followed by a lipolytic test on Nutrient Agar Fat (NAL) medium and a bioremediation test using oil palm leaf midrib extract at concentrations of 0.1%, 0.5%, and 1%. The results showed that there were nine types of colonies with three dominant bacteria, namely Candida spp. (95 colonies), Streptomyces sp. (11 colonies), and Aspergillus spp. (4 colonies). The three bacteria were shown to be lipolytically active, as evidenced by the formation of a clear zone in the NAL medium. Supplementation with oil palm frond extract significantly increased colony growth, with the highest yield at a concentration of 1% (>360 colonies/15 mL). ANOVA analysis showed differences in degradation effectiveness between colonies, with Aspergillus spp. exhibiting higher activity than other colonies. These findings confirm that the use of indigenous lipolytic bacteria in combination with oil palm frond extract can be a sustainable bioremediation strategy to reduce oil and fat content in LCPKS.
References
Abdul Karim, dan. (2019). Isolasi dan Uji Bakteri Lipolitik dalam Mendegradasi Minyak Pada Limbah Cair Kelapa Sawit di Kebun Marihat, Pematang Siantar Isolation and Testing of Lipolityc Bacteria in Oil Degradation of Palm Oil Mill Efluent (POME) at Marihat Pematang Siantar. Jurnal Ilmiah Biologi UMA (JIBIOMA), 1(2), 44–52. http://jurnalmahasiswa.uma.ac.id/index.php/jibioma
Al-Otibi, F., Al-Zahrani, R. M., & Marraiki, N. (2022). The crude oil biodegradation activity of Candida strains isolated from oil-reservoirs soils in Saudi Arabia. Scientific Reports, 12(1), 10708. https://doi.org/10.1038/s41598-022-14836-0
Bestari, N. C., & Suharjono. (2015). Uji Kualitatif dan Kuantitatif Isolat Bakteri Lipolitik dari Limbah Cair Pabrik Pengolahan Ikan Kecamatan Muncar, Banyuwangi. Jurnal Biotropika, 3(3), 151–155.
Boukaew, S., Cheirsilp, B., Yossan, S., Khunjan, U., Petlamul, W., & Prasertsan, P. (2022). Utilization of palm oil mill effluent as a novel substrate for the production of antifungal compounds by Streptomyces philanthi RM-1-138 and evaluation of its efficacy in suppression of three strains of oil palm pathogen. Journal of Applied Microbiology, 132(3), 1990–2003. https://doi.org/10.1111/jam.15304
Elyza, F., Gofar, N., & Munawar, M. (2016). Identifikasi dan Uji Potensi Bakteri Lipolitik Dari Limbah SBE (Spent Bleaching Earth) Sebagai Agen Bioremediasi. Jurnal Ilmu Lingkungan, 13(1), 12. https://doi.org/10.14710/jil.13.1.12-18
Fibriana, F., Upaichit, A., & Cheirsilp, B. (2022). Statistical Optimization for Cost-Effective Production of Yeast-Bacterium Cell-Bound Lipases Using Blended Oily Wastes and Their Potential Applications in Biodiesel Synthesis and Wastewater Bioremediation. Fermentation, 8(8), 411. https://doi.org/10.3390/fermentation8080411
Giraldo, L., Gómez-Granados, F., & Moreno-Piraján, J. C. (2023). Biodiesel Production Using Palm Oil with a MOF-Lipase B Biocatalyst from Candida Antarctica: A Kinetic and Thermodynamic Study. International Journal of Molecular Sciences, 24(13), 10741. https://doi.org/10.3390/ijms241310741
Harahap, A. A., Hanafi, N. D., Tafsin, M., & Umar, S. (2020). Substitusi rumput Lapang Dengan Pelepah Daun Kelapa Sawit Fermentasi Menggunakan Mikroorganisme Lokal Terhadap Kecernaan Nutrien dan Total Digestible Nutrient Pada Sapi Jantan Peranakan. Jurnal Ilmiah Peternakan Terpadu, 8(2), 47. https://doi.org/10.23960/jipt.v8i2.p47-52
Hassaine, A., & Bordjiba, O. (2019). Removal of hydrocarbons from liquid media by Aspergillus niger van Tieghem. Acta Ecologica Sinica, 39(4), 300–305. https://doi.org/10.1016/j.chnaes.2018.11.009
Hastuti, U. S. (2015). Penuntun Praktikum Mikrobiologi untuk Program S2 Biologi (Pertama). UMM Press.
Ilmannafian, A. G., Lestari, E., & Khairunisa, F. (2020). Pengolahan Limbah Cair Pabrik Kelapa Sawit dengan Metode Filtrasi dan Fitoremediasi Menggunakan Tanaman Eceng Gondok (Eichhornia Crassipes). Jurnal Teknologi Lingkungan, 21(2), 244–253. https://doi.org/10.29122/jtl.v21i2.4012
Kamaruddin, M. A., Ismail, N., Kuen, T. H., & Alrozi, R. (2018). Sustainable treatment of palm oil mill effluent (POME) by using Pectin and chitosan in jar test protocol - sequential comparison. International Journal of Integrated Engineering, 10(9), 163–169. https://doi.org/10.30880/ijie.2018.10.09.012
Khairani, K. M. (2023). Isolasi Dan Identifikasi Bakteri Lipolitik dari Limbah Cair Kelapa SawiT (Elaeis quineensis Jacq.). BIOEDUSAINS: Jurnal Pendidikan Biologi Dan Sains, 6(1), 1–13. https://bnr.bg/post/101787017/bsp-za-balgaria-e-pod-nomer-1-v-buletinata-za-vota-gerb-s-nomer-2-pp-db-s-nomer-12
Lanan, F. A. B. M., Selvarajoo, A., Sethu, V., & Arumugasamy, S. K. (2021). Utilisation of natural plant-based fenugreek (Trigonella foenum-graecum) coagulant and okra (Abelmoschus escluentus) flocculant for palm oil mill effluent (POME) treatment. Journal of Environmental Chemical Engineering, 9(1), 104667. https://doi.org/10.1016/j.jece.2020.104667
Ode Sumarlin, L., Faturrahman, ., & Yadial Chalid, S. (2019). Potential of Solid Oil Palm Waste as an Antribrowning Repellent of Aedes Aegypti. Jurnal Ilmu Pertanian Indonesia, 24(2), 117–126. https://doi.org/10.18343/jipi.24.2.117
Pratama, H. A. (2012). Pengelolaan Limbah Padat Industri Minyak Kelapa Sawit. Jurnal Teknologi Pengelolaan Limbah, 16(1), 1–14.
Rachmadona, N., Quayson, E., Amoah, J., Alfaro‐Sayes, D. A., Hama, S., Aznury, M., Kondo, A., & Ogino, C. (2021). Utilizing palm oil mill effluent (POME) for the immobilization of Aspergillus oryzae whole‐cell lipase strains for biodiesel synthesis. Biofuels, Bioproducts and Biorefining, 15(3), 804–814. https://doi.org/10.1002/bbb.2202
Resi, D. N., Missa, H., Djalo, A., & Ndukang, S. (2025). Isolasi Dan Karakterisasi Bakteri Lipolitik Dari Tanah Tempat Pembuangan Sementara Untuk Aplikasi Bioremediasi. JURNAL MEDIA INFORMATIKA [ JUMIN ]. 7(1), 195–203.
Rizky, M., Fitri, R., Hastuti, U., & Prabaningtyas, S. (2018). Identifikasi Uji Kemampuan Hidrolisis Lemak Dan Penentuan Indeks Zona Bening Asam Laktat Pada Bakteri Dalam Wadi Makanan Traditional Kalimantan Tengah. Bionature, 18. https://doi.org/10.35580/bionature.v18i2.6137
Rizky, M. Y. (2017). Identifikasi dan uji kemampuan hidrolisis lemak pada bakteri lipolitik yang diisolasi dari wadi. Universitas Negeri Malang.
Singh, R. P., Ibrahim, M. H., Esa, N., & Iliyana, M. S. (2010). Composting of waste from palm oil mill: A sustainable waste management practice. Reviews in Environmental Science and Biotechnology, 9(4), 331–344. https://doi.org/10.1007/s11157-010-9199-2
Soumeya, S., Allaoueddine, B., & Hocine, A.-K. (2022). Biodegradation of used motor oil by Streptomyces ginkgonis KM-1–2, isolated from soil polluted by waste oils in the region of Azzaba (Skikda-Algeria). Journal of Biotechnology, 349, 1–11. https://doi.org/10.1016/j.jbiotec.2022.03.006
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 Muhammad Nor, Noor Hujjatusnaini, Ridha Nirmalasari
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
