Penapisan Fitokimia, Fitur Antioksidan, dan Uji Toksisitas pada Ekstrak Metanol Kubis Ungu (Brassica oleracea var. capitata L.)

Authors

  • Selina Alvionica Universitas Tarumanagara
  • Siufui Hendrawan Universitas Tarumanagara
  • Frans Ferdinal Universitas Tarumanagara

DOI:

https://doi.org/10.33394/bioscientist.v13i3.16405

Keywords:

kubis ungu, fitokimia, fenolik, antioksidan, toksisitas

Abstract

This study aims to analyze the phytochemical content, antioxidant properties and toxicity of methanolic extract of red cabbage originating from West Java, Indonesia. The study comprised in vitro assays, which included phytochemical screening, determination of total phenolic level, and antioxidant activity tests. The toxicity was tested using Brine Shrimp Lethality Test (BSLT). The screening results revealed the presence of various secondary metabolites in the extract. The total phenolic content of red cabbage was 29.8 mgGAE/g. The antioxidant potential was evaluated through the FRAP (Ferric Reducing Antioxidant Power) and ABTS (2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) assays, yielding IC50 values of 13.42 µg/mL and 21.28 µg/mL, respectively; both were classified as having a strong antioxidant activity. The BSLT toxicity test showed an LC50 value of 153.87 µg/mL. These findings suggest that red cabbage is rich in beneficial phytochemicals and acts a strong natural antioxidants source. Moreover, it demonstrates potential for future development as a standardized herbal medicine with antioxidant properties and an affordable price.

References

Ahmed, M., Ji, M., Qin, P., Gu, Z., Liu, Y., Sikandar, A., Iqbal, M. F., & Javeed, A. (2019). Phytochemical screening, total phenolic and flavonoids contents and antioxidant activities of Citrullus colocynthis L. and Cannabis sativa L. Appl. Ecol. Environ. Res, 17(3), 6961–6979.

Apak, R., Özyürek, M., Güçlü, K., & Çapanoʇlu, E. (2016). Antioxidant activity/capacity measurement. 1. Classification, physicochemical principles, mechanisms, and electron transfer (ET)-based assays. Journal of Agricultural and Food Chemistry, 64(5), 997–1027. https://doi.org/10.1021/acs.jafc.5b04739

Aprian, F. D., Syamsudin, S., Zaidan, S., Susanti, D., & Hakim, Z. R. (2023). BSLT Toxicity Test Of Temulawak Ethanol Extract (Curcuma Xanthorrhiza) From Five Different Regions And Determination Of Curcumin And Xanthorhizol Compound Levels. OPSearch: American Journal of Open Research, 2(12), 828–838.

Ashfaq, F., Butt, M. S., Bilal, A., Tehseen, S., & Suleria, H. A. R. (2020). Comparative Assessment of Free Radical Scavenging Ability of Green and Red Cabbage Based on Their Antioxidant Vitamins and Phytochemical Constituents. Current Bioactive Compounds, 16(8), 1231–1241. https://doi.org/10.2174/1573407216666200127130014

Benzie, I. F. F., & Devaki, M. (2017). The ferric reducing/antioxidant power (FRAP) assay for non-enzymatic antioxidant capacity: Concepts, procedures, limitations and applications. Measurement of Antioxidant Activity and Capacity: Recent Trends and Applications, 77–106. https://doi.org/10.1002/9781119135388.ch5

Bibi Sadeer, N., Montesano, D., Albrizio, S., Zengin, G., & Mahomoodally, M. F. (2020). The Versatility of Antioxidant Assays in Food Science and Safety—Chemistry, Applications, Strengths, and Limitations. Antioxidants, 9(8), 709. https://doi.org/10.3390/antiox9080709

Bocso, N.-S., & Butnariu, M. (2022). The biological role of primary and secondary plants metabolites. Journal of Nutrition and Food Processing, 5(3), 1–7.

Borges, C. V., Junior, S. S., Ponce, F. S., & Lima, G. P. P. (2018). Agronomic Factors Influencing Brassica Productivity and Phytochemical Quality. Brassica Germplasm - Characterization, Breeding and Utilization. https://doi.org/10.5772/intechopen.74732

Budaraga, I. K., & Putra, D. P. (2021). Analysis antioxidant IC50 liquid smoke of cocoa skin with several purification methods. IOP Conference Series: Earth and Environmental Science, 757(1). https://doi.org/10.1088/1755-1315/757/1/012053

Burda, N., Protska, V., Fedosov, A., Kuznetsova, M., Zhuravel, I., Dababneh, M. F., Budanova, L., & Dobrovolnyi, O. (2021). The study of volatile fractions of Cabbage leaves (Brassica oleracea L. convar. capitata (L.) Alef. var. alba DC.) and determination of its antibacterial and antifungal activity:(TJPS-2020-0151). Thai Journal of Pharmaceutical Sciences (TJPS), 45(4).

Chaiyasut, C., Sivamaruthi, B. S., Pengkumsri, N., Sirilun, S., Peerajan, S., Chaiyasut, K., & Kesika, P. (2016). Anthocyanin Profile and Its Antioxidant Activity of Widely Used Fruits, Vegetables, And Flowers in Thailand. Asian Journal of Pharmaceutical and Clinical Research, 9(6), 218. https://doi.org/10.22159/ajpcr.2016.v9i6.14245

Chauhan, E. S., Tiwari, A., & Singh, A. (2016). Phytochemical screening of red cabbage (Brassica oleracea) powder and juice-A comparative study. Journal of Medicinal Plants Studies, 4(5), 196–199.

Chaves, N., Santiago, A., & Alías, J. C. (2020). Quantification of the antioxidant activity of plant extracts: analysis of sensitivity and hierarchization based on the method used. Antioxidants, 9(1), 76. https://doi.org/10.3390/antiox9010076

Cruz, A. B., Pitz, H. da S., Veber, B., Bini, L. A., Maraschin, M., & Zeni, A. L. B. (2016). Assessment of bioactive metabolites and hypolipidemic effect of polyphenolic-rich red cabbage extract. Pharmaceutical Biology, 54(12), 3033–3039. https://doi.org/10.1080/13880209.2016.1200633

Damayanti, P. G., Mustofa, A., & Karyantina, M. (2023). Aktivitas Antioksidan Nata dengan Substrat Dami Nangka (Artocarpus heterophyllus Lam) dan Kubis Ungu (Brassica oleracea L var. capitata). AGRITEKNO: Jurnal Teknologi Pertanian, 12(2), 124–134. https://doi.org/10.30598/jagritekno.2023.12.2.124

Eseberri, I., Trepiana, J., Léniz, A., Gómez-García, I., Carr-Ugarte, H., González, M., & Portillo, M. P. (2022). Variability in the Beneficial Effects of Phenolic Compounds: A Review. Nutrients, 14(9), 1925. https://doi.org/10.3390/nu14091925

Ghareaghajlou, N., Hallaj-Nezhadi, S., & Ghasempour, Z. (2021). Red cabbage anthocyanins: Stability, extraction, biological activities and applications in food systems. Food Chemistry, 365, 130482.

Gultom, D. K., Saraswati, I., & Sasikirana, W. (2021). Penetapan Kandungan Fenolik Total dan Uji Aktivitas Antioksidan dengan Metode DPPH (2, 2-difenil-1-pikrilhidrazil) Fraksi Etil Asetat Ekstrak Etanolik Kubis Ungu (Brassica oleraceae var. capitata L). Generics: Journal of Research in Pharmacy, 1(2), 79–87.

Haris, N. H., Nugroho, T., Utomo, A. W., & Yora Nindita. (2021). The effect of red cabbage extract to serum MDA levels in rats after maximum physical activity. Indonesian Journal of Pharmacology and Therapy, 2(3), 97–103. https://doi.org/10.22146/ijpther.1117

Hasanah, N. (2018). Uji Toksisitas Ekstrak Etanol Daun Kubis (Brassica Oleracea Var Capitata L) Dengan Metode Brine Shrimp Lethality Test (Bslt). Edu Dharma Journal: Jurnal Penelitian Dan Pengabdian Masyarakat, 2(1), 81–91.

Jumina, J., Siswanta, D., Zulkarnain, A. K., Triono, S., Priatmoko, P., Yuanita, E., Fatmasari, N., & Nursalim, I. (2019). Development of C-Arylcalix[4]resorcinarenes and C-Arylcalix[4]pyrogallolarenes as Antioxidant and UV-B Protector. Indonesian Journal of Chemistry, 19(2), 273. https://doi.org/10.22146/ijc.26868

Maheshwaran, L., Nadarajah, L., Senadeera, S. P. N. N., Ranaweera, C. B., Chandana, A. K., & Pathirana, R. N. (2024). Phytochemical Testing Methodologies and Principles for Preliminary Screening/ Qualitative Testing. Asian Plant Research Journal, 12(5), 11–38. https://doi.org/10.9734/aprj/2024/v12i5267

Mansour, K. A., Moustafa, S. F., & Abdelkhalik, S. M. (2021). High-resolution uplc-ms profiling of anthocyanins and flavonols of red cabbage (Brassica oleracea l. var. capitata f. rubra dc.) cultivated in egypt and evaluation of their biological activity. Molecules, 26(24). https://doi.org/10.3390/molecules26247567

Marsoul, A., Ijjaali, M., Elhajjaji, F., Taleb, M., Salim, R., & Boukir, A. (2020). Phytochemical screening, total phenolic and flavonoid methanolic extract of pomegranate bark (Punica granatum L): Evaluation of the inhibitory effect in acidic medium 1 M HCl. Materials Today: Proceedings, 27(xxxx), 3193–3198. https://doi.org/10.1016/j.matpr.2020.04.202

Meirista, I., Mariska, R. P., & Fahrul, M. (2025). Uji Aktivitas Antioksidan dan Penentuan Nilai SPF Sediaan Eyeshadow Cream Ekstrak Kubis Ungu (Brassica oleracea L. Var. Capitata f. rubra). Jurnal Pengembangan Ilmu Pengetahuan, 6(1).

Minich, D. M. (2019). A Review of the Science of Colorful, Plant-Based Food and Practical Strategies for “Eating the Rainbow.” Journal of Nutrition and Metabolism, 2019, 1–19. https://doi.org/10.1155/2019/2125070

Nguyen, N. H. K., Le Ngoc, T., PhanThiKieu, L., Tran Thanh, T., & Mai Huynh, C. (2020). Bioactive compounds from red cabbage by microwave-assisted extraction: Anthocyanins, total phenolic compounds and the antioxidant activity. Asian Life Sci, 12, 172–184.

Pallawagau, M., Yanti, N. A., Jahiding, M., Kadidae, L. O., Asis, W. A., & Hamid, F. H. (2019). Penentuan Kandungan Fenolik Total Liquid Volatile Matter dari Pirolisis Kulit Buah Kakao dan Uji Aktivitas Antifungi terhadap Fusarium oxysporum. Alchemy Jurnal Penelitian Kimia, 15(1), 165. https://doi.org/10.20961/alchemy.15.1.24678.165-176

Panchal Mital, D., & Jha, C. V. (2021). Qualitative and quantitative phytochemical screening of three plants stem bark and leaves from sapotaceae family. Int. J. Multidiscip. Educ. Res, 6(8), 1–6.

Rizal, K. R., & Anzani, R. (2025). Pengaruh Variasi Emulgator Terhadap Formulasi dan Stabilitas Fisik Body Scrub Ekstrak Kubis Ungu (Brassica Oleracea L.). SITAWA: Jurnal Farmasi Sains Dan Obat Tradisional, 4(1), 61–75.

Rumpf, J., Burger, R., & Schulze, M. (2023). Statistical evaluation of DPPH, ABTS, FRAP, and Folin-Ciocalteu assays to assess the antioxidant capacity of lignins. International Journal of Biological Macromolecules, 233, 123470. https://doi.org/10.1016/j.ijbiomac.2023.123470

Sabdoningrum, E. K., Hidanah, S., Chusniati, S., & Soeharsono. (2021). Characterization and Phytochemical Screening of Meniran (Phyllanthus niruri Linn) Extract’s Nanoparticles Used Ball Mill Method. Pharmacognosy Journal, 13(6), 1568–1572. https://doi.org/10.5530/pj.2021.13.200

Shah, P., & Modi, H. A. (2015). Comparative Study of DPPH, ABTS and FRAP Assays for Determination of Antioxidant Activity. International Journal for Research in Applied Science & Engineering Technology (IJRASET), 3(6), 636–641.

Shaikh, J. R., & Patil, M. (2020). Qualitative tests for preliminary phytochemical screening: An overview. International Journal of Chemical Studies, 8(2), 603–608. https://doi.org/10.22271/chemi.2020.v8.i2i.8834

Sharma, M., & Kaushik, P. (2021). Vegetable phytochemicals: An update on extraction and analysis techniques. Biocatalysis and Agricultural Biotechnology, 36, 102149. https://doi.org/10.1016/j.bcab.2021.102149

Shi, L., Zhao, W., Yang, Z., Subbiah, V., & Suleria, H. A. R. (2022). Extraction and characterization of phenolic compounds and their potential antioxidant activities. Environmental Science and Pollution Research, 29(54), 81112–81129.

Souza, A. de O., Bessa, D. H. R. F., Fernandes, C. C., Pereira, P. S., Martins, C. H. G., & Miranda, M. L. D. (2020). Phytochemical screening of extracts from Spiranthera odoratissima A. St.-Hil. (Rutaceae) leaves and their in vitro antioxidant and anti-Listeria monocytogenes activities. Acta Scientiarum. Biological Sciences, 42, e51881. https://doi.org/10.4025/actascibiolsci.v42i1.51881

Sukweenadhi, J., Yunita, O., Setiawan, F., Kartini, Siagian, M. T., Danduru, A. P., & Avanti, C. (2020). Antioxidant activity screening of seven Indonesian herbal extract. Biiodiversitas, 21(5), 2062–2067.

Sun, J., Liu, B., Rustiami, H., Xiao, H., Shen, X., & Ma, K. (2024). Mapping Asia Plants: Plant Diversity and a Checklist of Vascular Plants in Indonesia. Plants, 13(16), 2281. https://doi.org/10.3390/plants13162281

Susanti, R. E. E., Nurjanah, A., Safitri, R. E., & A’yun, Q. (2019). Pemanfaatan Ekstrak Kubis Ungu (Brassica Oleraceae) Sebagai Indikator Warna Pada Analisis Hidrokuinon. Akta Kimia Indonesia, 4(2), 95. https://doi.org/10.12962/j25493736.v4i2.5134

Tajalli, F., Saeedi, M., & Malekabadi, A. V. (2020). Anticancer and antioxidant effects of red cabbage on three cancerous cell lines and comparison with a normal cell line (HFF-3). Journal of Genes and Cells, 6(1), 12–20.

Tena, N., Martín, J., & Asuero, A. G. (2020). State of the Art of Anthocyanins: Antioxidant Activity, Sources, Bioavailability, and Therapeutic Effect in Human Health. Antioxidants, 9(5), 451. https://doi.org/10.3390/antiox9050451

Twaij, B. M., & Hasan, M. N. (2022). Bioactive Secondary Metabolites from Plant Sources: Types, Synthesis, and Their Therapeutic Uses. International Journal of Plant Biology, 13(1), 4–14. https://doi.org/10.3390/ijpb13010003

Utami, Y. P., Jariah, A., & Mustarin, R. (2023). Determination of UV-Vis Spectrophotometry with Differential pH on Total Anthocyanin Levels of Ethanol Extract of Cordyline fruticosa (L.) A. Cheval Leaves. Pharmaceutical Reports, 2(1), 10–14. https://doi.org/10.33096/pharmrep.v2i1.232

Vega-Galvez, A., Gomez-Perez, L. S., Zepeda, F., Vidal, R. L., Grunenwald, F., Mejías, N., Pasten, A., Araya, M., & Ah-Hen, K. S. (2023). Assessment of Bio-Compounds Content, Antioxidant Activity, and Neuroprotective Effect of Red Cabbage (Brassica oleracea var. Capitata rubra) Processed by Convective Drying at Different Temperatures. Antioxidants, 12(9), 1789. https://doi.org/10.3390/antiox12091789

Waghulde, S., Kale, M. K., & Patil, V. (2020). Brine Shrimp Lethality Assay of the Aqueous and Ethanolic Extracts of the Selected Species of Medicinal Plants. 2, 47. https://doi.org/10.3390/ecsoc-23-06703

Waghulde, S., Khan, N. A., Gorde, N., Kale, M., Naik, P., & Yewale, R. P. (2018). Comparative Antimicrobial Activity Study of Brassica oleceracea. 22nd International Electronic Conference on Synthetic Organic Chemistry, 64. https://doi.org/10.3390/ecsoc-22-05662

Wołosiak, R., Drużyńska, B., Derewiaka, D., Piecyk, M., Majewska, E., Ciecierska, M., Worobiej, E., & Pakosz, P. (2022). Verification of the conditions for determination of antioxidant activity by abts and dpph assays—a practical approach. Molecules, 27(1). https://doi.org/10.3390/molecules27010050

Zafar, I., Hussain, A. I., Fatima, T., Abdullah Alnasser, S. M., & Ahmad, A. (2022). Inter-Varietal Variation in Phenolic Profile, Sugar Contents, Antioxidant, Anti-Proliferative and Antibacterial Activities of Selected Brassica Species. Applied Sciences, 12(12), 5811. https://doi.org/10.3390/app12125811

Zakwan, M., Sutriana, A., Nurliana, Asmilia, N., Ammar, M., & Novianti, A. N. (2023). Toxicity Test of Flavonoid Compounds from Ethyl Acetate Extract of Malacca Leaves with Brine Shrimp Lethality Test. Jurnal Medik Veteriner, 6(3), 326–330. https://doi.org/10.20473/jmv.vol6.iss3.2023.326-330

Zayed, A., Sheashea, M., Kassem, I. A. A., & Farag, M. A. (2023). Red and white cabbages: An updated comparative review of bioactives, extraction methods, processing practices, and health benefits. Critical Reviews in Food Science and Nutrition, 63(24), 7025–7042.

Zehiroglu, C., & Ozturk Sarikaya, S. B. (2019). The importance of antioxidants and place in today’s scientific and technological studies. Journal of Food Science and Technology, 56(11), 4757–4774. https://doi.org/10.1007/s13197-019-03952-x

Zhao, Y., Yue, Z., Zhong, X., Lei, J., Tao, P., & Li, B. (2020). Distribution of primary and secondary metabolites among the leaf layers of headed cabbage (Brassica oleracea var. capitata). Food Chemistry, 312, 126028. https://doi.org/10.1016/j.foodchem.2019.126028

Downloads

Published

2025-09-28

How to Cite

Alvionica, S., Hendrawan, S., & Ferdinal, F. (2025). Penapisan Fitokimia, Fitur Antioksidan, dan Uji Toksisitas pada Ekstrak Metanol Kubis Ungu (Brassica oleracea var. capitata L.). Bioscientist : Jurnal Ilmiah Biologi, 13(3), 2390–2404. https://doi.org/10.33394/bioscientist.v13i3.16405

Issue

Section

Articles