Ekplorasi Senyawa Ekstrak Etanol Rumput Belulang (Eleusine indica (L.) Gaertn) Menggunakan LC-MS/MS-QTOF Serta Uji Aktivitas Anti-Diare: In-Vivo

Authors

  • Rizki Rahmadi Pratama Program Studi Sarjana Farmasi, Fakultas Farmasi, Universitas Islam Kalimantan Muhammad Arsyad Al-Banjari http://orcid.org/0000-0002-5275-7211
  • Andre Septianto Program Studi Sarjana Farmasi, Fakultas Farmasi, Universitas Islam Kalimantan Muhammad Arsyad Al-Banjari
  • Said Muhammad Al-Hasyimi Program Studi Sarjana Farmasi, Fakultas Farmasi, Universitas Islam Kalimantan Muhammad Arsyad Al-Banjari
  • Herliana Herliana Program Studi Sarjana Farmasi, Fakultas Farmasi, Universitas Islam Kalimantan Muhammad Arsyad Al-Banjari
  • Hasniah Hasniah Program Studi Sarjana Farmasi, Fakultas Farmasi, Universitas Islam Kalimantan Muhammad Arsyad Al-Banjari
  • Faisal Akhmal Muslikh Program Studi Sarjana Farmasi, Fakultas Farmasi, Universitas Hang Tuah, Indonesia

DOI:

https://doi.org/10.33394/bioscientist.v12i2.14192

Keywords:

antidiarrheal, Eleusine indica, LC-MS/MS-QToF, secondary metabolites

Abstract

Diarrhea is one of the diseases caused by symptoms of intestinal tract infections due to various microorganisms. Goosegrass (Eleusine indica (L.) Gaertn) is a wild weed that has long been used as a traditional medicine, attributed to its phytochemical compounds such as tannins, alkaloids, flavonoids. This study aims to evaluate the antidiarrheal effects of Eleusine indica extract by comparing six treatment groups: a healthy group, a negative control group (Na-CMC), a positive control group (Loperamide 2 mg), and three variations of the extract dosage. Observations were made over 360 minutes at 30-minute intervals, using a fecal consistency scale from 1 (solid) to 5 (liquid). LC-MS/QToF analysis identified 25 secondary metabolites in the extract, including flavonoids (Schaftoside, Orientin, Vitexin, Tricin), phenolic compounds, and terpenoids. In vivo testing showed that the third dose of Eleusine indica grass extract had the most optimal antidiarrheal effect among the three doses, as evidenced by its ability to reduce diarrhea frequency, decrease fecal weight, shorten diarrhea duration, and improve fecal consistency, approaching the effectiveness of Loperamide 2 mg as a positive control. These findings suggest the potential of Eleusine indica extract as a promising natural antidiarrheal agent.

References

Bohlmann, F., Mahanta, P. K., Natu, A. A., King, R. M., & Robinson, H. (1978). New germacranolides from Isocarpha species. Phytochemistry, 17(3), 471–474. https://doi.org/https://doi.org/10.1016/S0031-9422(00)89341-3

Bouaziz, M., Simmonds, M. S. J., Grayer, R. J., Kite, G. C., & Damak, M. (2001). Flavonoids from Hyparrhenia hirta Stapf (Poaceae) growing in Tunisia. Biochemical Systematics and Ecology, 29(8), 849–851. https://doi.org/https://doi.org/10.1016/S0305-1978(01)00028-X

Collins, F. W. (1989). Oat phenolics: avenanthramides, novel substituted N-cinnamoylanthranilate alkaloids from oat groats and hulls. Journal of Agricultural and Food Chemistry, 37(1), 60–66. https://doi.org/10.1021/jf00085a015

Cometa, J. C., Faith, L., Mancol, R., Yen, A. N., & Katherine, J. (2020). ACE Inhibitory Activity and Functional Group Analysis of Solvent- Partitioned Fractions of Eleusine indica. 7(1), 57–65.

Davyt, D., Entz, W., Fernandez, R., Mariezcurrena, R., Mombrú, A. W., Saldaña, J., Domínguez, L., Coll, J., & Manta, E. (1998). A New Indole Derivative from the Red Alga Chondria atropurpurea. Isolation, Structure Determination, and Anthelmintic Activity. Journal of Natural Products, 61(12), 1560–1563. https://doi.org/10.1021/np980114c

De Melo, G. O., Muzitano, M. F., Legora-Machado, A., Almeida, T. A., De Oliveira, D. B., Kaiser, C. R., Koatz, V. L. G., & Costa, S. S. (2005). C-glycosylflavones from the aerial parts of Eleusine indica inhibit LPS-induced mouse lung inflammation. Planta Medica, 71(4), 362–363. https://doi.org/10.1055/s-2005-864104

Fauzi, R., Fatmawati, A., & Emelda, E. (2020). Efek Antidiare Ekstrak Etanol Daun Kelor (Moringa oleifera L.) Pada Mencit Putih Jantan. Pharmaceutical Journal of Indonesia, 6, 35–39. https://doi.org/10.21776/ub.pji.2020.006.01.6

Gunstone, F. D., & Subbarao, R. (1967). New tropical seed oils. Part I. conjugated trienoic and tetraenoic acids and their oxo derivatives in the seed oils of Chrysobalanus Icaco and Parinarium Laurinum. Chemistry and Physics of Lipids, 1(4), 349–359. https://doi.org/https://doi.org/10.1016/0009-3084(67)90012-6

Iberahim, R., Yaacob, W. A., & Ibrahim, N. (2015). Phytochemistry, cytotoxicity and antiviral activity of Eleusine indica (sambau). AIP Conference Proceedings, 1678(1), 30013. https://doi.org/10.1063/1.4931234

Iryanto, A., Joko, T., & Raharjo, M. (2021). Literature Review : Faktor Risiko Kejadian Diare Pada Balita Di Indonesia. Jurnal Kesehatan Lingkungan, 11, 1–7. https://doi.org/10.47718/jkl.v11i1.1337

Jeffreys, J. A. D. (1970). The alkaloids of perennial rye-grass (Lolium perenne L.). Part IV. Isolation of a new base, perlolyrine; the crystal structure of its hydrobromide dihydrate, and the synthesis of the base. Journal of the Chemical Society C: Organic, 8, 1091–1103. https://doi.org/10.1039/J39700001091

Khuzhaev, V. U., Zhalolov, I. Z., Levkovich, M. G., Aripova, S. F., & Shashkov, A. S. (2004). Alkaloids of Arundo donax L. Russian Chemical Bulletin, 53(8), 1765–1767. https://doi.org/10.1007/s11172-005-0033-x

Kementrian Kesehatan RI., 2018. Profil Kesehatan Indonesia 2018. URL: http://www.kemkes.go.id. Diakses tanggal 3 Februari 2024

Kementrian Kesehatan RI., 2019. Profil Kesehatan Indonesia 2019. URL: https://www.kemkes.go.id/id/profil-kesehatan-indonesia-2019.pdf. Diakes tanggal 3 Februari 2024

Kementrian Kesehatan RI., 2022. Laporan Kinerja 2022. URL https://p2pm.kemkes.go.id/storage/informasipublik/content/GHwE3BiLbOrvZZPKY1Pm91BIRWqzE4-metaTGFwa2luIFAyUE0gMjAyMi5wZGY=-.pdf Diakes tanggal; 3 Februari 2024

Lee, E. M., Lee, S. S., Chung, B. Y., Cho, J.-Y., Lee, I. C., Ahn, S. R., Jang, S. J., & Kim, T. H. (2010). Pancreatic Lipase Inhibition by C-Glycosidic Flavones Isolated from Eremochloa ophiuroides. In Molecules (Vol. 15, Issue 11, pp. 8251–8259). https://doi.org/10.3390/molecules15118251

Machida, K., Sasaki, H., Iijima, T., & Kikuchi, M. (2002). Studies on the Constituents of Lonicera Species. XVII. New Iridoid Glycosides of the Stems and Leaves of Lonicera japonica THUNB. Chemical and Pharmaceutical Bulletin, 50(8), 1041–1044. https://doi.org/10.1248/cpb.50.1041

Magadelin, B. J., Sinthuja, S. A., & Ahamed, S. J. (2023). Phytochemical Screening and Biological activities of Eleusine indica leaf extract. 15(3), 444–449.

Orji, C. E., Madu, N. C., Okwelogu, I. S., Chekwube, J., Nedum, C. H., Ogbue, C. O., Olli, V. N., & Nnaemeka, J. (2024). Anti-diarrheal effects of ethanol leaf extract of Eleusine Indica in Castor Oil induced diarrhea using mice model.

Powell, R. G., Plattner, R. D., Yates, S. G., Clay, K., & Leuchtmann, A. (1990). Ergobalansine, a New Ergot-Type Peptide Alkaloid Isolated from Cenchrus echinatus (Sandbur Grass) Infected with Balansia obtecta, and Produced in Liquid Cultures of B. obtecta and Balansia cyperi. Journal of Natural Products, 53(5), 1272–1279. https://doi.org/10.1021/np50071a021

Pradana, D. L. C., & Batubara, U. M. (2015). Identifikasi Senyawa Bioaktif Dan Uji Aktivitas Antibakteri Infusa Eleusine Indica Dan Daun Morinda Citrifolia Pada Bakteri Staphylococcus Aureus Secara In Vitro. 662–669.

Pratama, R. R., Sholikhah, I., Sukardiman, Sahu, R. K., & Widyowati, R. (2023). Phytochemical Compounds Identification From 70% Ethanol Extract of Arcangelesia Flava (L.) Merr Stems Using LC-MS/MS and In-Silico Molecular Docking Approach as Inhibitor Interleukin-1β. Pharmacognosy Journal, 15, 528–534. https://doi.org/10.5530/pj.2023.15.114

Rahayuningsih, N., Assyifa, S., & Rahmiyani, I. (2021). Antidiarrheal Activity Test of White Pomegranate (Punica granatum L.) Infusion on Swiss Webster Male with Oleum Ricini Method. Jurnal Ilmu Kefarmasian Indonesia, 19, 62. https://doi.org/10.35814/jifi.v19i1.943

Septiani, D., Hastuti, E. D., & Darmanti, S. (2019). Efek Alelokimia Ekstrak Daun Babandotan (Ageratum Conyzoides L.) Terhadap Kandungan Pigmen Fotosintetik Dan Pertumbuhan Gulma Rumput Belulang (Eleusine Indica (L.) Gaertn). Buletin Anatomi Dan Fisiologi, 4(1).https://doi.org/10.9767/Bcrec.5346.

Sholikha, M., Arini, N., & Sains, I. (2023). Efek Antidiare Ekstrak Etanol Akar Senggani ( Melastoma Malabathricum L .) Pada Mencit Swiss Webster Jantan. 50–58.

Sitarek, P., Merecz-Sadowska, A., Kowalczyk, T., Wieczfinska, J., Zajdel, R., & Śliwiński, T. (2020). Potential Synergistic Action of Bioactive Compounds from Plant Extracts against Skin Infecting Microorganisms. International Journal of Molecular Sciences, 21(14). https://doi.org/10.3390/ijms21145105

Subehan, Usia, T., Kadota, S., & Tezuka, Y. (2005). Constituents of Zingiber aromaticum and Their CYP3A4 and CYP2D6 Inhibitory Activity. Chemical and Pharmaceutical Bulletin, 53(3), 333–335. https://doi.org/10.1248/cpb.53.333

Sukor, N. S. M., Zakri, Z. H. M., Rasol, N. E., & Salim, F. (2023). Annotation and Identification of Phytochemicals from Eleusine indica Using High-Performance Liquid Chromatography Tandem Mass Spectrometry: Databases-Driven Approach. Molecules (Basel, Switzerland), 28(7). https://doi.org/10.3390/molecules28073111

Tava, A., Berardo, N., & Odoardi, M. (1991). Composition of essential oil of tall fescue. Phytochemistry, 30(5), 1455–1458. https://doi.org/https://doi.org/10.1016/0031-9422(91)84185-U

Ueyama, Y., Arai, T., & Hashimoto, S. (1991). Volatile constituents of ethanol extracts of Hierochloe odorata L. var. pubescens Kryl. Flavour and Fragrance Journal, 6, 63–68. https://api.semanticscholar.org/CorpusID:94245813

Watanabe, M. (1999). Antioxidative Phenolic Compounds from Japanese Barnyard Millet (Echinochloa utilis) Grains. Journal of Agricultural and Food Chemistry, 47(11), 4500–4505. https://doi.org/10.1021/jf990498s

Wenzl, P., Chaves, A. L., Mayer, J. E., Rao, I. M., & Nair, M. G. (2000). Roots of nutrient-deprived Brachiaria species accumulate 1,3-di-O-trans-feruloylquinic acid. Phytochemistry, 55(5), 389–395. https://doi.org/https://doi.org/10.1016/S0031-9422(00)00350-2

World Health Organization (WHO), 2019. Monitoring Health for the SDGs, Sustainable Development Goals URL: https://www.who.int/ Diakes tanggal; 3 Februari 2024

Yagi, S., Babiker, R., Tzanova, T., & Schohn, H. (2016). Chemical composition, antiproliferative, antioxidant and antibacterial activities of essential oils from aromatic plants growing in Sudan. Asian Pacific Journal of Tropical Medicine, 9(8), 763–770. https://doi.org/https://doi.org/10.1016/j.apjtm.2016.06.009

Yates, S. G., Plattner, R. D., & Garner, G. B. (1985). Detection of ergopeptine alkaloids in endophyte-infected, toxic Ky-31 tall fescue by mass spectrometry/mass spectrometry. Journal of Agricultural and Food Chemistry, 33(4), 719–722. https://doi.org/10.1021/jf00064a038

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Published

2024-12-29

How to Cite

Pratama, R. R., Septianto, A., Al-Hasyimi, S. M., Herliana, H., Hasniah, H., & Muslikh, F. A. (2024). Ekplorasi Senyawa Ekstrak Etanol Rumput Belulang (Eleusine indica (L.) Gaertn) Menggunakan LC-MS/MS-QTOF Serta Uji Aktivitas Anti-Diare: In-Vivo. Bioscientist : Jurnal Ilmiah Biologi, 12(2), 2518–2530. https://doi.org/10.33394/bioscientist.v12i2.14192

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Vol. 12 No. 2 (2024): December

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